Widely used methods for determining bacterial numbers are the plate count method and spectrophotometric (turbidimetric) analysis. Beside that certain test kits are available dedicated for specific kinds of bacteria. Screening tests can be performed by using the ATP technique with a luminometer and specific sampling tubes as mikrocount® duo.
The designation SRB stands for Sulfate Reducing Bacteria. These are organisms which reduce sulfate to sulfide in the absence of oxygen. The most common organisms of this type associated with contamination of the industrial environment are found in the genera Desulfovibrio and Desulfotomaculum. When sulfide is liberated, it reacts with iron in the tubed culture medium to form iron sulfide, a black precipitate. The degree to which the medium blackens, along with the length of time it takes to change color, allows for an estimated count of sulfate reducers to be made.
We recommend Sulfate-reducing bacteria test kits from ECHA Microbiology.:
ATP is a molecule found in and around living cells, and as such it gives a direct measure of biological concentration and health. ATP is quantified by measuring the light produced through its reaction with the naturally occurring firefly enzyme luciferase using a luminometer. The amount of light produced is directly proportional to the amount of living organisms present in the sample. Results of ATP measurement can be read in a few seconds.
ATP test kits are offered from several suppliers, e.g.:
mikrocount® duo is a plastic slide coated on one side with TTCagar (bacterial growth) and on the other side with Rose-bengal-agar (yeast and mould growth). It is supplied with a transparent, shatterproof protective tube. Subsequent contamination is therefore prevented, and the results can be evaluated with the tube sealed, thus avoiding the risk of contamination to employees. mikrocount® duo dip slides enable simple sampling and evaluation of the results, even by personnel without any microbiological training. Special laboratory equipment is not necessary.more
Easy to use
Quick and convenient
Accurate and reliable
mikrocount® duo evaluation chart
The cultura® incubator is compact and versatile enough for almost any laboratory or manufacturing setting. The built in tray has room to hold up to 18 mikrocount® duo dip slide samples. A transparent door allows for viewing of the contents without removing samples from the incubator.
The adjustable temperature is pre-set by the manufacturer to maintain 30°C, an optimum temperature for incubating mikrocount® dip slides. Results for bacteria are available after 24 to 48 hours. The detection of yeast and moulds takes slightly longer (72 hours).